Exploring Pectin: From Extraction Challenges to Pharmaceutical Applications

Ali Assifaoui

doi:10.63095/NBSEH.25.010299

Authors

Ali Assifaoui Unité Mixte de Recherche Procédés Alimentaires et Microbiologiques, L’Institut Agro, Université Bourgogne Europe (https://ror.org/00g700j37), INRAE, 21000, Dijon, France UFR Sciences de Santé, Pharmaceutical Technology Department, Bd Jeanne d’Arc, 21079, Dijon, France

DOI:

https://doi.org/10.63095/NBSEH.25.010299

Keywords:

Pectin extraction, Pharmaceutical applications, Drug delivery systems

Abstract

This review examines recent advances and uses of pectin, a versatile polysaccharide, with a focus on its extraction from agricultural by-products and its emerging applications in the pharmaceutical industry. Pectin, mainly sourced from citrus peels and apple pomace, is a complex heteropolysaccharide with significant structural diversity, which influences its functional properties. The review discusses various extraction methods, including conventional acidic extraction and environmentally responsible techniques such as microwave-assisted, ultrasound-assisted, subcritical water, and supercritical CO₂ extraction. These methods are evaluated for their efficiency and environmental impact. In addition, the review highlights the functional properties of pectin, such as its roles as a stabilizer, thickener, and gelling agent, which are important in pharmaceutical applications. The pharmaceutical uses of pectin receive particular attention, with emphasis on its role in drug delivery systems, including floating systems and encapsulation technologies. The review also addresses the challenges and future prospects of pectin use, underscoring its potential to replace other hydrocolloids given its cost-effectiveness and functional benefits.

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References

Voragen, A. G. J., Pilnik, W., Thibault, J.-F., Axelos, M. A. V., & Renard, C. M. G. C., 1995. Pectins. In: Stephen, A. M. (Ed.) Food Polysaccharides and Their Applications, 10. (New York: Dekker), pp. 287–339. Available at: https://prodinra.inra.fr/record/115643

Pagliaro, M., Ciriminna, R., Fidalgo, A., Delisi, R., & Ilharco, L., 2016, Pectin production and global market. Agro Food Industry Hi Tech 27(5), pp. 17–20. Available at: https://www.qualitas1998.net/pagliaro/Pectin-production-and-global-market.pdf

Da Silva, J. L., & Rao, M. A., 2006, Pectins: structure, functionality, and uses. In: Stephen, A. M. (ed.) Food Polysaccharides and Their Applications. (New York: Marcel Dekker), p. 35. Available at: https://books.google.co.ma/books?id=UInMBQAAQBAJ

Rehman, Z. U., Salariya, A. M., Habib, F., & Shah, W. H., 2004, Utilization of mango peels as a source of pectin. Journal of the Chemical Society of Pakistan 26, pp. 73–76. Available at: https://jcsp.org.pk/ArticleUpload/1298-5869-1-PB.pdf

Kermani, Z. J., Shpigelman, A., Kyomugasho, C., Van Buggenhout, S., Ramezani, M., Van Loey, A. M., & Hendrickx, M. E., 2014, The impact of extraction with a chelating agent under acidic conditions on the cell wall polymers of mango peel. Food Chemistry 161, 199–207. https://doi.org/10.1016/j.foodchem.2014.03.131

Swamy G. J., & Muthukumarappan K., 2017, Optimization of continuous and intermittent microwave extraction of pectin from banana peels. Food Chemistry 220, 108–114. https://doi.org/10.1016/j.foodchem.2016.09.197

Minjares-Fuentes R., Femenia A., Garau M. C., Meza-Velázquez J. A., Simal S., & Rosselló C., 2014, Ultrasound-assisted extraction of pectins from grape pomace using citric acid: A response surface methodology approach. Carbohydrate Polymers 106, 179–189. https://doi.org/10.1016/j.carbpol.2014.02.013

Colodel C., Vriesmann L. C., Teófilo R. F., & de Oliveira Petkowicz C. L., 2020, Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv. Chardonnay) pomace, a winery waste. International Journal of Biological Macromolecules 161, 204–213. https://doi.org/10.1016/j.ijbiomac.2020.05.272.

Maran J. P., Sivakumar V., Thirugnanasambandham K., & Sridhar R., 2014, Microwave assisted extraction of pectin from waste Citrullus lanatus fruit rinds. Carbohydrate Polymers 101, 786–791. https://doi.org/10.1016/j.carbpol.2013.09.062

Santos, J. D. G., Espeleta, A. F., Branco, A., & de Assis, S. A., 2013, Aqueous extraction of pectin from sisal waste. Carbohydrate Polymers 92(2), 1997-2001. https://doi.org/10.1016/j.carbpol.2012.11.089

Liew, S. Q., Teoh, W. H., Tan, C. K., Yusoff, R., & Ngoh, G. C., 2018, Subcritical water extraction of low methoxyl pectin from pomelo (Citrus grandis (L.) Osbeck) peels. International Journal of Biological Macromolecules 116, 128-135. https://doi.org/10.1016/j.ijbiomac.2018.05.013

Yoo S.-H., Lee B.-H., Lee H., Lee S., Bae I.Y., Lee H.G., Fishman M.L., Chau H.K., Savary B.J., & Hotchkiss A.T. Jr., 2012, Structural characteristics of pumpkin pectin extracted by microwave heating, Journal of Food Science 77(11), C1169–C1173. https://doi.org/10.1111/j.1750-3841.2012.02960.x

Willats, W. G., Knox, J. P., & Mikkelsen, J. D., 2006, Pectin: new insights into an old polymer are starting to gel. Trends in Food Science & Technology 17(3), 97-104. https://doi.org/10.1016/j.tifs.2005.10.008

Voragen A. G. J., Coenen G.-J., Verhoef R. P., & Schols H. A., 2003. Pectins: structure and function. In: Voragen A. G. J., Schols H. A., Visser R. G. F. (Eds), Advances in Pectin and Pectinase Research, 47–70. (Dordrecht: Springer Netherlands). https://doi.org/10.1007/978-94-017-0331-4_7

Ropartz D., & Ralet M.-C., 2020. Pectin structure. In: Kontogiorgos V. (Ed), Pectin: Technological and Physiological Properties, 17–38. (Cham: Springer) https://doi.org/10.1007/978-3-030-53421-9

Naqash F., Masoodi F. A., Rather S. A., Wani S. M., & Gani A., 2017, Emerging concepts in the nutraceutical and functional properties of pectin—A review. Carbohydrate Polymers 168, 227–239. https://doi.org/10.1016/j.carbpol.2017.03.058

Mills K. E., & Mackerras D., 2016. Does daily consumption of pectin lower cholesterol concentration? A systematic review and meta-analysis. Journal of Nutrition & Intermediary Metabolism 4, 11. https://doi.org/10.1016/j.jnim.2015.12.189

Munarin F., Tanzi M. C., & Petrini P., 2012, Advances in biomedical applications of pectin gels. International Journal of Biological Macromolecules 51(4), 681–689. https://doi.org/10.1016/j.ijbiomac.2012.07.002

Li D. Q., Li J., Dong H. L., Li X., Zhang J. Q., Ramaswamy S., & Xu F., 2021, Pectin in biomedical and drug delivery applications: A review. International Journal of Biological Macromolecules 185, 49–65. https://doi.org/10.1016/j.ijbiomac.2021.06.088

Zhang, W., Xu, P., & Zhang, H., 2015, Pectin in cancer therapy: A review. Trends in Food Science & Technology 44(2), 258-271. https://doi.org/10.1016/j.tifs.2015.04.001

Assifaoui, A., Hayrapetyan, G., Gallery, C., & Agoda-Tandjawa, G., 2024, Exploring techno-functional properties, synergies, and challenges of pectins: A review. Carbohydrate Polymer Technologies and Applications 7, 100496. https://doi.org/10.1016/j.carpta.2024.100496

Brummer Y., Cui S.W., 2005, Understanding carbohydrate analysis, in: Cui S.W. (Ed.), Food carbohydrates: Chemistry, physical properties, and applications, (New York: CRS Press), pp. 67–104. https://doi.org/10.1201/9780203485286

Garna, H., Mabon, N., Wathelet, B., & Paquot, M., 2004, New method for a two-step hydrolysis and chromatographic analysis of pectin neutral sugar chains. Journal of agricultural and food chemistry 52(15), 4652-4659. https://doi.org/10.1021/jf049647j

Deng, C., O’Neill, M. A., & York, W. S., (2006). Selective chemical depolymerization of rhamnogalacturonans. Carbohydrate Research, 341(4), 474-484. https://doi.org/10.1016/j.carres.2005.12.004

Novosel'Skaya, I. L., Voropaeva, N. L., Semenova, L. N., & Rashidova, S. S., 2000, Trends in the science and applications of pectins. Chemistry of Natural Compounds 36(1), 1-10. https://doi.org/10.1007/BF02234898

Morris, G. A., & Ralet, M. C., 2012, The effect of neutral sugar distribution on the dilute solution conformation of sugar beet pectin. Carbohydrate Polymers 88(4), 1488-1491. https://doi.org/10.1016/j.carbpol.2012.02.020

Bochek, A. M., Zabivalova, N. M., & Petropavlovskii, G. A., (2001). Determination of the esterification degree of polygalacturonic acid. Russian Journal of Applied Chemistry 74(5), 796-799. https://doi.org/10.1023/A:1012701219447

Chatjigakis, A. K., Pappas, C., Proxenia, N., Kalantzi, O., Rodis, P., & Polissiou, M., 1998, FT-IR spectroscopic determination of the degree of esterification of cell wall pectins from stored peaches and correlation to textural changes. Carbohydrate Polymers 37(4), 395-408. https://doi.org/10.1016/S0144-8617(98)00057-5

Gnanasambandam, R., & Proctor, A. J. F. C., 2000, Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. Food Chemistry 68(3), 327-332. https://doi.org/10.1016/S0308-8146(99)00191-0

Gary,W., Purification and characterization of pectin acetylesterase from orange peel. Phytochemistry 30(2), 445-449. https://doi.org/10.1016/0031-9422(91)83701-L

Kim, M. J., Ju, H. K., Kim, Y., Yoo, S. H., & Kim, Y. S., 2016, Effects of amidation and/or methylesterification of pectin on aroma release at different calcium concentration. Food Hydrocolloids 52, 343-349. https://doi.org/10.1016/j.foodhyd.2015.07.006

Sinitsya, A., Čopı́ková, J., Prutyanov, V., Skoblya, S., & Machovič, V., 2000, Amidation of highly methoxylated citrus pectin with primary amines. Carbohydrate Polymers 42(4), 359-368. https://doi.org/10.1016/S0144-8617(99)00184-8

Alba, K., Dimopoulou, M., & Kontogiorgos, V., (2021). Baobab polysaccharides as emulsifiers. LWT, 144, 111235. https://doi.org/10.1016/j.lwt.2021.111235

Zheng, C., Zhang, Z., Ding, N., Huang, B., Zhang, G., & Fei, P., 2022, Synthesis of amidated pectin with amino acid using ultra-low temperature enzymatic method and its evaluation of fat mimic characteristics. Food Hydrocolloids 125, 107454. https://doi.org/10.1016/j.foodhyd.2021.107454

Phillips G.O., & Williams P.A. (Eds.), 2009, Handbook of hydrocolloids, 2nd ed., (Cambridge: Elsevier). (Woodhead Publishing Series in Food Science, Technology and Nutrition). p. 274-297. https://www.yumpu.com/en/user/registration?utm_content=reg-pdf-download&goto=dv63233637

Löfgren, C., Guillotin, S., Evenbratt, H., Schols, H., & Hermansson, A. M., 2005, Effects of calcium, pH, and blockiness on kinetic rheological behavior and microstructure of HM pectin gels. Biomacromolecules 6(2), 646-652. www.doi.org/10.1021/bm049619

Guillotin, S. E., Bakx, E. J., Boulenguer, P., Schols, H. A., & Voragen, A. G. J., 2007, Determination of the degree of substitution, degree of amidation and degree of blockiness of commercial pectins by using capillary electrophoresis. Food Hydrocolloids 21(3), 444-451. https://doi.org/10.1016/j.foodhyd.2006.05.003

Ström, A., Ribelles, P., Lundin, L., Norton, I., Morris, E. R., & Williams, M. A., 2007, Influence of pectin fine structure on the mechanical properties of calcium− pectin and acid − pectin gels. Biomacromolecules 8(9), 2668-2674. www.doi.org/10.1021/bm070192r

Ngouémazong, D. E., Jolie, R. P., Cardinaels, R., Fraeye, I., Van Loey, A., Moldenaers, P., & Hendrickx, M., 2012, Stiffness of Ca2+-pectin gels: combined effects of degree and pattern of methylesterification for various Ca2+ concentrations. Carbohydrate Research 348, 69-76. https://doi.org/10.1016/j.carres.2011.11.011

Ralet, M. C., Williams, M. A., Tanhatan-Nasseri, A., Ropartz, D., Quéméner, B., & Bonnin, E., 2012, Innovative enzymatic approach to resolve homogalacturonans based on their methylesterification pattern. Biomacromolecules 13(5), 1615-1624. www.doi.org/10.1021/bm300329r

Ralet, M. C., Crépeau, M. J., & Bonnin, E., 2008. Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin. Phytochemistry 69(9), 1903-1909. https://doi.org/10.1016/j.phytochem.2008.03.008

May, C. D., 1990, Industrial pectins: Sources, production and applications. Carbohydrate Polymers 12(1), 79-99. https://doi.org/10.1016/0144-8617(90)90105-2

Oshima, T., Kato, K., & Imaizumi, T., 2021, Effects of blanching on drying characteristics, quality, and pectin nanostructures of dried cut-persimmons. LWT -Technology and Science Food 143, 111094. https://doi.org/10.1016/j.lwt.2021.111094

Cui, J., Zhao, C., Feng, L., Han, Y., Du, H., Xiao, H., & Zheng, J., 2021, Pectins from fruits: Relationships between extraction methods, structural characteristics, and functional properties. Trends in Food Science & Technology 110, 39-54. https://doi.org/10.1016/j.tifs.2021.01.077

Wandee, Y., Uttapap, D., & Mischnick, P., 2019, Yield and structural composition of pomelo peel pectins extracted under acidic and alkaline conditions. Food Hydrocolloids 87, 237-244. https://doi.org/10.1016/j.foodhyd.2018.08.017

Wang, K., Hua, X., Yang, R., Kang, J., & Zhang, W., 2014, Hydrodynamic behavior and gelling properties of sunflower head pectin in the presence of sodium salts. Food Hydrocolloids 36, 238-244. https://doi.org/10.1016/j.foodhyd.2013.09.011

Vriesmann, L. C., Teófilo, R. F., & de Oliveira Petkowicz, C. L., 2011, Optimization of nitric acid-mediated extraction of pectin from cacao pod husks (Theobroma cacao L.) using response surface methodology. Carbohydrate Polymers 84(4), 1230-1236. https://doi.org/10.1016/j.carbpol.2011.01.009

Sudhakar, D. V., & Maini, S. B., 2000, Isolation and characterization of mango peel pectins. Journal of Food Processing and Preservation 24(3), 209-227. https://doi.org/10.1111/j.1745-4549.2000.tb00414.x

Ralet, M. C., & Thibault, J. F., 1994, Extraction and characterisation of very highly methylated pectins from lemon cell walls. Carbohydrate Research 260(2), 283-296. https://doi.org/10.1016/0008-6215(94)84046-6

Kratchanova M., Yanakieva I., Kussovski V., & Kratchanova M., 2012, Isolation, characterization and modification of citrus pectins. Journal of BioScience and Biotechnology 1(3), 223–233. Available at: https://agris.fao.org/search/en/providers/122436/records/6759a5760ce2cede71c8b836

Kumar, A., & Chauhan, G. S., 2010, Extraction and characterization of pectin from apple pomace and its evaluation as lipase (steapsin) inhibitor. Carbohydrate Polymers 82(2), 454-459. https://doi.org/10.1016/j.carbpol.2010.05.001

Zhang, H., Chen, J., Li, J., Yan, L., Li, S., Ye, X., Liu, D., Ding, T., Linhardt, R. J., Orfila, C., & Chen, S., 2018, Extraction and characterization of RG-I enriched pectic polysaccharides from mandarin citrus peel. Food Hydrocolloids 79, 579–586. https://doi.org/10.1016/j.foodhyd.2017.12.002

Feng, L., Zhou, Y., Ashaolu, T. J., Ye, F., & Zhao, G., 2019, Physicochemical and rheological characterization of pectin-rich fraction from blueberry (Vaccinium ashei) wine pomace. International Journal of Biological Macromolecules 128, 629-637. https://doi.org/10.1016/j.ijbiomac.2019.01.166

Kratchanova, M., Pavlova, E., & Panchev, I., 2004, The effect of microwave heating of fresh orange peels on the fruit tissue and quality of extracted pectin. Carbohydrate Polymers 56(2), 181-185. https://doi.org/10.1016/j.carbpol.2004.01.009

Fishman, M. L., Chau, H. K., Hoagland, P. D., & Hotchkiss, A. T., 2006, Microwave-assisted extraction of lime pectin. Food Hydrocolloids 20(8), 1170-1177. https://doi.org/10.1016/j.foodhyd.2006.01.002

Su, D. L., Li, P. J., Quek, S. Y., Huang, Z. Q., Yuan, Y. J., Li, G. Y., & Shan, Y., 2019, Efficient extraction and characterization of pectin from orange peel by a combined surfactant and microwave assisted process. Food Chemistry 286, 1-7. https://doi.org/10.1016/j.foodchem.2019.01.200

Seixas, F. L., Fukuda, D. L., Turbiani, F. R., Garcia, P. S., Petkowicz, C. L. D. O., Jagadevan, S., & Gimenes, M. L., 2014, Extraction of pectin from passion fruit peel (Passiflora edulis f. flavicarpa) by microwave-induced heating. Food Hydrocolloids 38, 186-192. https://doi.org/10.1016/j.foodhyd.2013.12.001

Hosseini, S. S., Khodaiyan, F., & Yarmand, M. S., 2016, Optimization of microwave assisted extraction of pectin from sour orange peel and its physicochemical properties. Carbohydrate Polymers 140, 59-65. https://doi.org/10.1016/j.carbpol.2015.12.051

Sun, D., Chen, X., & Zhu, C., 2020, Physicochemical properties and antioxidant activity of pectin from hawthorn wine pomace: A comparison of different extraction methods. International Journal of Biological Macromolecules 158, 1239-1247. https://doi.org/10.1016/j.ijbiomac.2020.05.052

Gerschenson, L. N., Fissore, E. N., Rojas, A. M., Encalada, A. M. I., Zukowski, E. F., & Coelho, R. A. H., 2021, Pectins obtained by ultrasound from agroindustrial by-products. Food Hydrocolloids 118, 106799. https://doi.org/10.1016/j.foodhyd.2021.106799

García-Marino, M., Rivas-Gonzalo, J. C., Ibáñez, E., & García-Moreno, C., 2006, Recovery of catechins and proanthocyanidins from winery by-products using subcritical water extraction. Analytica Chimica Acta 563(1-2), 44-50. https://doi.org/10.1016/j.aca.2005.10.054

Herrero, M., Temirzoda, T. N., Segura-Carretero, A., Quirantes, R., Plaza, M., & Ibañez, E., 2011, New possibilities for the valorization of olive oil by-products. Journal of Chromatography A 1218(42), 7511-7520. https://doi.org/10.1016/j.chroma.2011.04.053

Ko, M. J., Cheigh, C. I., Cho, S. W., & Chung, M. S., 2011, Subcritical water extraction of flavonol quercetin from onion skin. Journal of Food Engineering 102(4), 327-333. https://doi.org/10.1016/j.jfoodeng.2010.09.008

Aliakbarian, B., Fathi, A., Perego, P., & Dehghani, F., 2012. Extraction of antioxidants from winery wastes using subcritical water. The Journal of Supercritical Fluids, 65, 18-24. https://doi.org/10.1016/j.supflu.2012.02.022

Ueno, H., Tanaka, M., Hosino, M., Sasaki, M., & Goto, M., 2008, Extraction of valuable compounds from the flavedo of Citrus junos using subcritical water. Separation and Purification Technology 62(3), 513-516. https://doi.org/10.1016/j.seppur.2008.03.004

Wang, X., Chen, Q., & Lü, X., 2014, Pectin extracted from apple pomace and citrus peel by subcritical water. Food Hydrocolloids 38, 129-137. https://doi.org/10.1016/j.foodhyd.2013.12.003

Chen, H. M., Fu, X., & Luo, Z. G., 2015, Properties and extraction of pectin-enriched materials from sugar beet pulp by ultrasonic-assisted treatment combined with subcritical water. Food Chemistry 168, 302-310. https://doi.org/10.1016/j.foodchem.2014.07.078

Ruthes, A. C., Martínez-Abad, A., Tan, H. T., Bulone, V., & Vilaplana, F., 2017, Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes. Green Chemistry 19(8), 1919-1931. https://doi.org/10.1039/C6GC03473J

De Melo, M. M. R., Silvestre, A. J. D., & Silva, C. M., 2014, Supercritical fluid extraction of vegetable matrices: Applications, trends and future perspectives of a convincing green technology. The Journal of Supercritical Fluids 92, 115-176. https://doi.org/10.1016/j.supflu.2014.04.007

Cao, X., & Ito, Y., 2003, Supercritical fluid extraction of grape seed oil and subsequent separation of free fatty acids by high-speed counter-current chromatography. Journal of Chromatography A 1021(1-2), 117-124. https://doi.org/10.1016/j.chroma.2003.09.001

Pavlić, B., Vidović, S., Vladić, J., Radosavljević, R., & Zeković, Z., 2015, Isolation of coriander (Coriandrum sativum L.) essential oil by green extractions versus traditional techniques. The Journal of Supercritical Fluids 99, 23-28. https://doi.org/10.1016/j.supflu.2015.01.029

Passos, C. P., Silva, R. M., Da Silva, F. A., Coimbra, M. A., & Silva, C. M., 2009, Enhancement of the supercritical fluid extraction of grape seed oil by using enzymatically pre-treated seed. The Journal of Supercritical Fluids 48(3), 225-229. https://doi.org/10.1016/j.supflu.2008.11.001

Martínez, M. L., Mattea, M. A., & Maestri, D. M., 2008, Pressing and supercritical carbon dioxide extraction of walnut oil. Journal of Food Engineering 88(3), 399-404. https://doi.org/10.1016/j.jfoodeng.2008.02.026

Rivas, M. Á., Casquete, R., de Guía Córdoba, M., Benito, M. J., Hernández, A., Ruiz-Moyano, S., & Martín, A., 2021, Functional properties of extracts and residual dietary fibre from pomegranate (Punica granatum L.) peel obtained with different supercritical fluid conditions. LWT -Technology and Science Food 145, 111305. https://doi.org/10.1016/j.lwt.2021.111305

Hayrapetyan, G., Trchounian, K., Buon, L., Noret, L., Pinel, B., Lagrue, J., & Assifaoui, A., 2023, Sequential extraction of high-value added molecules from grape pomaces using supercritical fluids with water as a co-solvent. RSC Sustainability 1(8), 2014-2023. https://doi.org/10.1039/D3SU00183K

Dehnad, D., Jafari, S. M., & Afrasiabi, M., 2016, Influence of drying on functional properties of food biopolymers: From traditional to novel dehydration techniques. Trends in Food Science & Technology 57, 116-131. https://doi.org/10.1016/j.tifs.2016.09.002

Schmidt, I., Cousin, F., Huchon, C., Boué, F., & Axelos, M. A., 2009, Spatial structure and composition of polysaccharide − protein complexes from small angle neutron scattering. Biomacromolecules 10(6), 1346-1357. www.doi.org/10.1021/bm801147j

Assifaoui, A., Lerbret, A., Uyen, H. T., Neiers, F., Chambin, O., Loupiac, C., & Cousin, F., 2015, Structural behaviour differences in low methoxy pectin solutions in the presence of divalent cations (Ca2+ and Zn2+): A process driven by the binding mechanism of the cation with the galacturonate unit. Soft Matter 11(3), 551-560. https://doi.org/10.1039/C4SM01839G

Alba, K., Bingham, R. J., Gunning, P. A., Wilde, P. J., & Kontogiorgos, V., 2018, Pectin conformation in solution. The Journal of Physical Chemistry B 122(29), 7286-7294. https://doi.org/10.1021/acs.jpcb.8b04790

Huang, X., Li, D., & Wang, L. J., 2017, Characterization of pectin extracted from sugar beet pulp under different drying conditions. Journal of Food Engineering 211, 1-6. https://doi.org/10.1016/j.jfoodeng.2017.04.022

Guo, Y., Wei, Y., Cai, Z., Hou, B., & Zhang, H., 2021, Stability of acidified milk drinks induced by various polysaccharide stabilizers: A review. Food Hydrocolloids 118, 106814. https://www.sciencedirect.com/journal/food-hydrocolloids

Rubinstein M., & Colby R.H., 2003, Polymer physics (Oxford: Oxford University Press). https://doi.org/10.1093/oso/9780198520597.001.0001

Wusigale, L. Liang, L. I., & Luo, Y., 2020, Casein and pectin: Structures, interactions, and applications. Trends in Food Science & Technology 97, 391-403. https://doi.org/10.1016/j.tifs.2020.01.027

Cooper, C. L., Dubin, P. L., Kayitmazer, A. B., & Turksen, S., 2005, Polyelectrolyte–protein complexes. Current Opinion in Colloid & Interface Science 10(1-2), 52-78. https://doi.org/10.1016/j.cocis.2005.05.007

Tolstoguzov, V., 2003, Some thermodynamic considerations in food formulation. Food Hydrocolloids 17(1), 1-23. https://doi.org/10.1016/S0268-005X(01)00111-4

Axelos, M. A. V., & Thibault, J. F., 1991, Influence of the substituents of the carboxyl groups and of the rhamnose content on the solution properties and flexibility of pectins. International Journal of Biological Macromolecules 13(2), 77-82. https://doi.org/10.1016/0141-8130(91)90052-V

Capel, F., Nicolai, T., Durand, D., Boulenguer, P., & Langendorff, V., 2006, Calcium and acid induced gelation of (amidated) low methoxyl pectin. Food Hydrocolloids 20(6), 901-907. https://doi.org/10.1016/j.foodhyd.2005.09.004

Grant, G. T., Morris, E. R., Rees, D. A., Smith, P. J., & Thom, D., 1973, Biological interactions between polysaccharides and divalent cations: the egg-box model. FEBS Letters 32(1), 195-198. https://doi.org/10.1016/0014-5793(73)80770-7

Lerbret, A., & Assifaoui, A., 2025, Binding of Fe2+ to Polygalacturonate: Structure of Dimers and Coordination Geometry. Biomacromolecules. https://doi.org/10.1021/acs.biomac.5c00120

Lerbret, A., & Assifaoui, A., 2022, How accurate is the egg-box model in describing the binding of calcium to polygalacturonate? A molecular dynamics simulation study. The Journal of Physical Chemistry B 126(48), 10206-10220. https://doi.org/10.1021/acs.jpcb.2c05374

Du Poset, A. M., Zitolo, A., Cousin, F., Assifaoui, A., & Lerbret, A., 2020, Evidence for an egg-box-like structure in iron (ii)–polygalacturonate hydrogels: a combined EXAFS and molecular dynamics simulation study. Physical Chemistry Chemical Physics 22(5), 2963-2977. https://doi.org/10.1039/C9CP04065J

Li, L., Fang, Y., Vreeker, R., Appelqvist, I., & Mendes, E., 2007, Reexamining the egg-box model in calcium− alginate gels with X-ray diffraction. Biomacromolecules 8(2), 464-468. https://doi.org/10.1021/bm060550a

Fang, Y., Al-Assaf, S., Phillips, G. O., Nishinari, K., Funami, T., Williams, P. A., & Li, L., 2007, Multiple steps and critical behaviors of the binding of calcium to alginate. The Journal of Physical Chemistry B 111(10), 2456-2462. https://doi.org/10.1021/jp0689870

Ventura, I., Jammal, J., & Bianco-Peled, H., 2013, Insights into the nanostructure of low-methoxyl pectin–calcium gels. Carbohydrate Polymers 97(2), 650-658. https://doi.org/10.1016/j.carbpol.2013.05.055

Donati, I., Benegas, J. C., & Paoletti, S., 2006, Polyelectrolyte study of the calcium-induced chain association of pectate. Biomacromolecules 7(12), 3439-3447. https://doi.org/10.1021/bm060164t

Cao, L., Lu, W., Mata, A., Nishinari, K., & Fang, Y., 2020, Egg-box model-based gelation of alginate and pectin: A review. Carbohydrate Polymers 242, 116389. https://doi.org/10.1016/j.carbpol.2020.116389

Celus, M., Kyomugasho, C., Van Loey, A. M., Grauwet, T., & Hendrickx, M. E., 2018, Influence of pectin structural properties on interactions with divalent cations and its associated functionalities. Comprehensive Reviews in Food Science and Food Safety 17(6), 1576-1594. https://doi.org/10.1111/1541-4337.12394

Williams, M.A.K., 2020, Pectin gelation and its assembly into functional materials, in : Pectin: Technological and physiological properties, pp. 125–148 (Cham: Springer International Publishing). https://doi.org/10.1007/978-3-030-53421-9_7

Borgogna, M., Skjåk-Bræk, G., Paoletti, S., & Donati, I., 2013, On the initial binding of alginate by calcium ions. The tilted egg-box hypothesis. The Journal of Physical Chemistry B 117(24), 7277-7282. https://doi.org/10.1021/jp4030766

Huynh, U. T., Lerbret, A., Neiers, F., Chambin, O., & Assifaoui, A., 2016, Binding of divalent cations to polygalacturonate: A mechanism driven by the hydration water. The Journal of Physical Chemistry B 120(5), 1021-1032. https://doi.org/10.1021/acs.jpcb.5b11010

Ashford, M., Fell, J., Attwood, D., Sharma, H., & Woodhead, P., 1994, Studies on pectin formulations for colonic drug delivery. Journal of Controlled Release 30(3), 225-232. https://doi.org/10.1016/0168-3659(94)90028-0

Sriamornsak, P., & Nunthanid, J., 1998, Calcium pectinate gel beads for controlled release drug delivery: I. Preparation and in vitro release studies. International Journal of Pharmaceutics 160(2), 207-212. https://doi.org/10.1016/S0378-5173(97)00310-4

Lara-Espinoza, C., Carvajal-Millán, E., Balandrán-Quintana, R., López-Franco, Y., & Rascón-Chu, A., 2018, Pectin and pectin-based composite materials: Beyond food texture. Molecules 23(4), 942. https://doi.org/10.3390/molecules23040942

Tian L., Scholte J., Borewicz K., van den Bogert B., Smidt H., Scheurink A.J.W., Gruppen H., & Schols H.A., 2016, Effects of pectin supplementation on the fermentation patterns of different structural carbohydrates in rats, Molecular Nutrition & Food Research 60(10), 2256–2266. https://doi.org/10.1002/mnfr.201600149

Assifaoui A., & Chambin O., 2020, Pectin as drug-release vehicle, in : Kontogiorgos V. (Ed.), Pectin: Technological and physiological properties, pp. 189–207 (Cham: Springer Nature). https://doi.org/10.1007/978-3-030-53421-9

Huynh U.T.D., Assifaoui A., & Chambin O., 2017, Pellets based on polyuronates: Relationship between gelation and release properties, Journal of Food Engineering 199, 27–35. https://doi.org/10.1016/j.jfoodeng.2016.12.004

Assifaoui, A., Bouyer, F., Chambin, O., & Cayot, P., 2013, Silica-coated calcium pectinate beads for colonic drug delivery. Acta Biomaterialia 9(4), 6218-6225. https://doi.org/10.1016/j.actbio.2012.11.031

Dhalleine, C., Assifaoui, A., Moulari, B., Pellequer, Y., Cayot, P., Lamprecht, A., & Chambin, O., 2011, Zinc-pectinate beads as an in vivo self-assembling system for pulsatile drug delivery. International Journal of Pharmaceutics 414(1-2), 28-34. https://doi.org/10.1016/j.ijpharm.2011.04.059

Assifaoui, A., Chambin, O., & Cayot, P., 2011, Drug release from calcium and zinc pectinate beads: Impact of dissolution medium composition. Carbohydrate Polymers 85(2), 388-393. https://doi.org/10.1016/j.carbpol.2011.02.037

Siripruekpong, W., Wiwattanapatapee, R., Chambin, O., & Assifaoui, A., 2025, Structural and mechanistic exploration in the development of floating drug delivery systems using calcium-pectinate gels with sodium bicarbonate as CO2 gas-forming agent. Journal of Drug Delivery Science and Technology 107, 106729. https://doi.org/10.1016/j.jddst.2025.106729

Sriamornsak, P., Sungthongjeen, S., & Puttipipatkhachorn, S., 2007, Use of pectin as a carrier for intragastric floating drug delivery: Carbonate salt contained beads. Carbohydrate Polymers 67(3), 436-445. https://doi.org/10.1016/j.carbpol.2006.06.013

Arora, S., Ali J., Ahuja A., Khar, R. K., & Baboota S., 2005, Floating drug delivery systems: a review, AAPS PharmSciTech 6, 47. https://doi.org/10.1208/pt060347

du Poset, A. M., Lerbret, A., Zitolo, A., Cousin, F., & Assifaoui, A., 2018, Design of polygalacturonate hydrogels using iron (II) as cross-linkers: A promising route to protect bioavailable iron against oxidation. Carbohydrate Polymers 188, 276-283. https://doi.org/10.1016/j.carbpol.2018.02.007

Heumann A., Assifaoui A., Da Silva Barreira D., Thomas C., Briandet R., Laurent J., Beney L., Lapaquette P., Guzzo J., & Rieu A., 2020, Intestinal release of biofilm-like microcolonies encased in calcium-pectinate beads increases probiotic properties of Lacticaseibacillus paracasei, npj Biofilms and Microbiomes 6(1), 44. https://doi.org/10.1038/s41522-020-00159-3

Maire du Poset, A., M. Börjesson, C. Rameau, C. Madeleine-Perdrillat, A. Lerbret, C. Loupiac, F. Cousin, & A. Assifaoui, 2020, Controlled loading and release of beta-lactoglobulin in calcium-polygalacturonate hydrogels. Biomacromolecules 21(4), 1417-1426. https://doi.org/10.1021/acs.biomac.9b01722

Ropartz, D., Lemoine, J., Giuliani, A., Bittebière, Y., Enjalbert, Q., Antoine, R., Dugourd, P., Ralet, M.C., & Rogniaux, H., 2014, Deciphering the structure of isomeric oligosaccharides in a complex mixture by tandem mass spectrometry: photon activation with vacuum ultra-violet brings unique information and enables definitive structure assignment, Analytica Chimica Acta 807, 84–95. https://doi.org/10.1016/j.aca.2013.11.018

Dong, X., Huang, Y., Cho, B.G., Zhong, J., Gautam, S., Peng, W., Williamson, S.D., Banazadeh, A., Torres-Ulloa, K.Y., & Mechref, Y., 2018, Advances in mass spectrometry-based glycomics, Electrophoresis 39(24), 3063–3081. https://doi.org/10.1002/elps.201800273

Mucha, E., Marianski, M., Xu, F.-F., Thomas, D.A., Meijer, G., von Helden, G., Seeberger, P.H., & Pagel, K., 2018, Unravelling the structure of glycosyl cations via cold-ion infrared spectroscopy, Nature Communications 9(1), 4174. https://doi.org/10.1038/s41467-018-06764-3

Bonnin, E., Garnier, C., & Ralet, M.C., 2014, Pectin-modifying enzymes and pectin-derived materials: applications and impacts, Applied Microbiology and Biotechnology 98(2), 519–532. https://doi.org/10.1007/s00253-013-5388-6

Morris, G.A., Ralet, M.C., Bonnin, E., Thibault, J.F., & Harding, S.E., 2010, Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin, Carbohydrate Polymers 82(4), 1161–1167. https://doi.org/10.1016/j.carbpol.2010.06.049

Levigne, S., Ralet, M.C., & Thibault, J.F., 2002, Characterisation of pectins extracted from fresh sugar beet under different conditions using an experimental design, Carbohydrate Polymers 49(2), 145–153. https://doi.org/10.1016/S0144-8617(01)00314-9

Ralet, M.C., Dronnet, V., Buchholt, H.C., & Thibault, J.F., 2001, Enzymatically and chemically de-esterified lime pectins: characterisation, polyelectrolyte behaviour and calcium binding properties, Carbohydrate Research 336(2), 117–125. https://doi.org/10.1016/S0008-6215(01)00248-8

Yapo, B.M., Lerouge, P., Thibault, J.F., & Ralet, M.C., 2007, Pectins from citrus peel cell walls contain homogalacturonans homogenous with respect to molar mass, rhamnogalacturonan I and rhamnogalacturonan II, Carbohydrate Polymers 69(3), 426–435. https://doi.org/10.1016/j.carbpol.2006.12.024

Bonnin, E., & Pelloux, J., 2020, Pectin degrading enzymes, in: Kontogiorgos, V. (Ed.), Pectin: Technological and physiological properties, pp. [à compléter] (Cham: Springer Nature). https://doi.org/10.1007/978-3-030-53421-9

Geerkens, C.H., Nagel, A., Just, K.M., Miller-Rostek, P., Kammerer, D.R., Schweiggert, R.M., & Carle, R., 2015, Mango pectin quality as influenced by cultivar, ripeness, peel particle size, blanching, drying, and irradiation, Food Hydrocolloids 51, 241–251. https://doi.org/10.1016/j.foodhyd.2015.05.022

Ramadan, M.F., & Moersel, J.T., 2007, Impact of enzymatic treatment on chemical composition, physicochemical properties and radical scavenging activity of goldenberry (Physalis peruviana L.) juice, Journal of the Science of Food and Agriculture 87(3), 452–460. https://doi.org/10.1002/jsfa.2728

Yapo, B.M., 2009, Pectin quantity, composition and physicochemical behaviour as influenced by the purification process, Food Research International 42(8), 1197–1202. https://doi.org/10.1016/j.foodres.2009.06.002

Reginatto, V., Amante, E.R., Gerhardy, K., Kunst, S., & Duran, N., 2009, Biodegradation and ecotoxicological assessment of pectin production wastewater, Journal of Environmental Sciences 21(11), 1613–1619. https://doi.org/10.1016/S1001-0742(08)62463-8

Bidault, O., Assifaoui, A., Champion, D., & Le Meste, M., 2005, Dielectric spectroscopy measurements of the sub-Tg relaxations in amorphous ethyl cellulose: a relaxation magnitude study, Journal of Non-Crystalline Solids 351(14–15), 1167–1178. https://doi.org/10.1016/j.jnoncrysol.2005.03.014

Roudaut, G., Simatos, D., Champion, D., Contreras-Lopez, E., & Le Meste, M., 2004, Molecular mobility around the glass transition temperature: a mini review, Innovative Food Science & Emerging Technologies 5(2), 127–134. https://doi.org/10.1016/j.ifset.2003.12.003

Roudaut, G., Farhat, I., Poirier-Brulez, F., & Champion, D., 2009, Influence of water, temperature and sucrose on dynamics in glassy starch-based products studied by low field 1H NMR, Carbohydrate Polymers 77(3), 489–495. https://doi.org/10.1016/j.carbpol.2009.01.029

Maxwell, A.S., Monnerie, L., & Ward, I.M., 1998, Secondary relaxation processes in polyethylene terephthalate–additive blends: 2. Dynamic mechanical and dielectric investigations, Polymer 39(26), 6851–6859. https://doi.org/10.1016/S0032-3861(98)00138-4