Biofilm Formation Revisited: Contrasting Effects of Natural and Industrial Orange Juices on Bacterial Biofilm Development

Ghita Radi Benjelloun; FatimaZahra Boutaleb; Zakaria Asbai; Mohamed Bennani; Assya Aharrar; Khadija Khataby; Fatima Hamadi; Ikrame Charef; Rajaa Tesse

doi:10.63095/NBSEH.25.279204

Authors

Ghita Radi Benjelloun Hassan II University of Casablanca (https://ror.org/001q4kn48), Laboratory of Biotechnology, Agri-Food, Materials and Environment, Team: Biotechnologies, Resource Valorisation, Quality, Health and Ecotoxicology, Faculty of Science and Technology Mohammedia, BP 146, Mohammedia 20650, Morocco
FatimaZahra Boutaleb Hassan II University of Casablanca (https://ror.org/001q4kn48), Laboratory of Biotechnology, Agri-Food, Materials and Environment, Team: Biotechnologies, Resource Valorisation, Quality, Health and Ecotoxicology, Faculty of Science and Technology Mohammedia, BP 146, Mohammedia 20650, Morocco
Zakaria Asbai Hassan II University of Casablanca (https://ror.org/001q4kn48), Laboratory of Biotechnology, Agri-Food, Materials and Environment, Team: Biotechnologies, Resource Valorisation, Quality, Health and Ecotoxicology, Faculty of Science and Technology Mohammedia, BP 146, Mohammedia 20650, Morocco
Mohamed Bennani Institut Pasteur Casablanca Morocco (https://ror.org/04yb4j419), Laboratory of Physico-Chemical Analysis of Water, Food and Environment, Casablanca 20250, Morocco
Assya Aharrar Laboratory of Microbial Biotechnology and Plants Protection. Biology Department. Sciences Faculty, Ibn Zohr University (https://ror.org/006sgpv47), Agadir, Morocco
Khadija Khataby Hassan II University of Casablanca (https://ror.org/001q4kn48), Laboratory of Biotechnology, Agri-Food, Materials and Environment, Team: Biotechnologies, Resource Valorisation, Quality, Health and Ecotoxicology, Faculty of Science and Technology Mohammedia, BP 146, Mohammedia 20650, Morocco
Fatima Hamadi Laboratory of Microbial Biotechnology and Plants Protection. Biology Department. Sciences Faculty, Ibn Zohr University (https://ror.org/006sgpv47), Agadir, Morocco
Ikrame Charef Hassan II University of Casablanca (https://ror.org/001q4kn48), Laboratory of Biotechnology, Agri-Food, Materials and Environment, Team: Biotechnologies, Resource Valorisation, Quality, Health and Ecotoxicology, Faculty of Science and Technology Mohammedia, BP 146, Mohammedia 20650, Morocco
Rajaa Tesse Hassan II University of Casablanca (https://ror.org/001q4kn48), Laboratory of Biotechnology, Agri-Food, Materials and Environment, Team: Biotechnologies, Resource Valorisation, Quality, Health and Ecotoxicology, Faculty of Science and Technology Mohammedia, BP 146, Mohammedia 20650, Morocco

DOI:

https://doi.org/10.63095/NBSEH.25.279204

Keywords:

Biofilm, Orange juice, Adhesion, XDLVO, Polysaccharide

Abstract

This study compares the biofilm-forming potential of Escherichia coli and Staphylococcus aureus acclimatized to two different conditions: natural orange juice and orange juice reconstituted and standardized from natural concentrate. The adhesion results predicted by the XDLVO model were compared with the experimental. Strains acclimatized to natural orange juice show significantly increased adhesion, unlike orange juice from the food industry, which considerably reduces adhesion. FTIR analyses reveal an increase in polysaccharides in strains exposed to natural juice. The results also showed the limitations of the predictive XDLVO approach, as experimental adhesion remains relatively high even when the ∆GTotal values are relatively larger and positive, which theoretically indicates weak adhesion. These results highlight the impact of bacterial acclimatization and the need to integrate biological interactions into the study of biofilm formation. They also require evaluating the link between the food environment and the behaviour of gut microbiota bacteria, with a view to preventing cross-contamination and improving food safety.

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